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| Registros recuperados: 20 | |
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| Rosa,Rosimeire Takaki; Napimoga,Marcelo Henrique; Höfling,José Francisco; Gonçalves,Reginaldo Bruno; Rosa,Edvaldo Antonio Ribeiro. |
| Twenty-one Streptococcus mutans strains were clustered by Multilocus Enzyme Electrophoresis (MLEE). Six isoenzymes showed strong infra-specific discriminatory power (M1P, MPI, PLP, NSP, GOT, and LAP). MLEE is a robust technique that may be used to explore clonal diversity of S. mutans isolates in epidemiological surveys. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Streptococcus mutans; Isoenzymes; Clonal variability. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100003 |
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| Coelho,Stefânia Vilas Boas; Rosa,Sttela Dellyzete Veiga Franco da; Fantazzini,Tatiana Botelho; Baute,Júlia Lima; Silva,Luciano Coutinho. |
| ABSTRACT Coffee is one of the main agricultural commodities in the country, and it is important to conservation of plant material for breeding programs. Cryopreservation is a promising alternative for preserving in the long-term the germplasm of species considered recalcitrant. However, studies should be performed to achieve maximum survival of seedlings after immersion in liquid nitrogen. The objective of this work was to find a cryopreservation protocol for storing seeds of Coffea canephora, studying two methods of cryopreservation, slow and fast cooling. Seeds were subjected to drying in silica gel up to the water content of 0.25 g g-1. In the first experiment, dried seeds were subjected to treatments of slow cooling at speeds of -1 ºC min-1,-3 ºC min-1... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Drying in silica gel; Recalcitrant seeds; Ice crystals; Isoenzymes; Physiological quality. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542018000600588 |
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| Silva, Lílian Abadia da; Sales, Juliana de Fátima; Oliveira, João Almir; Pinho, Édila Vilela de Resende Von; Santos, Heloisa Oliveira dos; Soares, Marcos Antônio. |
| This study aimed to investigate the desiccation tolerance of Rhamnidium elaeocarpum Reissek seeds through physiological and biochemical alterations. Fruit were collected from the municipality of Jaupaci, Goias State, Brazil, when the water content of their seeds had a 37% wet basis (w.b.) and the seeds were subsequently kept in a drying oven with air circulation at a temperature of 35ºC until a water content was reached of 20 to 12% (w.b.). The following parameters were evaluated: germination, germination speed index, emergence, emergence speed index, electrical conductivity and respiration rate. Furthermore, the electrophoretic profile of the isoenzymes: catalase, peroxidase and esterase, the enzymatic activities of endo-β-mannanase and α-amylase and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5.01.03.00-8 drying; Germination; Isoenzymes; Respiration rate; Heat-resistant proteins Produção e beneficiamento de sementes. |
| Ano: 2015 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19425 |
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| Bessega,Cecilia; Saidman,Beatriz Ofelia; Vilardi,Juan César. |
| In the present work, isoenzyme electrophoresis was used to analyze the variability and phenetic relationships among seven American species of genus Prosopis belonging to three different sections: P. argentina (Monilicarpa), P. glandulosa, P. velutina, P. flexuosa, P. ruscifolia, P. kuntzei (Algarobia), and P. reptans (Strombocarpa). The genetic variability in P. argentina, P. reptans, and P. kuntzei was significantly lower than in the rest of the species analyzed. The species belonging to different sections are highly differentiated, but the relationships retrieved among species belonging to the section Algarobia suggested that the series of this section are not natural groups. P. kuntzei is as differentiated from the remaining species of Algarobia as from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Prosopis; Isoenzymes; Genetic variability; Phenetic relationships. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200018 |
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| SAAVEDRA,CARMEN C. R.; NAPP,MARLY; REGULY,MARIA LUIZA; VALENTE,VERA LUCIA S. |
| Natural populations of Drosophila willistoni collected both in urban sites and in the wild, as control, were analysed with respect to their isoenzyme polymorphisms in six loci of enzyme systems, through horizontal electrophoresis. The first pool of populations was collected and analysed during the years 1987 and 1988, and the other during 1992 and 1993, in comparable seasons. The level of enzymatic polymorphism first detected was smaller in comparison to that observed four years later, suggesting an increase of variability along the time elapsed since the first sampling of this species, in the city of Porto Alegre. Our findings are suggestive of an increasing adjustment of the species to this new environment |
| Tipo: Journal article |
Palavras-chave: Drosophila willistoni; Urban populations; Isoenzymes. |
| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2001000000010 |
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| Araújo,J.V.; Junghans,T.G.; Alfenas,A.C.; Gomes,A.P.S.. |
| Extraction and isoenzyme analysis of four isolates of Arthrobotrys including A. musiformis, A. robusta and A. conoides were conducted. Among the 14 enzymes studied by starch gel electrophoresis, using morpholine-citrate as gel/electrode buffer, the following nine enzymes showed interpretable banding patterns: <!-- $MVD$:face("Symbol") --><FONT FACE="Symbol">a</font>-esterase, fumarase, hexokinase, isocitrate dehydrogenase, leucine aminopeptidase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphoglucomutase and phosphoglucoisomerase. All isolates studied displayed typical isoenzyme phenotypes for each species. Two isolates of A. conoides differed in their <!-- $MVD$:face("Symbol") --><FONT... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nematophagous fungi; Nematode-trapping fungus; Predacious fungi; Arthrobotrys; Isoenzymes; Electrophoresis. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000001 |
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| Cruz,Sara Michelly; Nery,Marcela Carlota; Von Pinho,Édila Vilela de Resende; Laia,Marcelo Luiz de. |
| The radish has been gaining importance in the Brazilian market due to its use as a green manure and cover crop, and also due to its potential for the production of biodiesel, however there are only two registered cultivars, which are morphologically very similar. Determination of genetic purity is a prerequisite in marketing seeds as it ensures uniformity of the crop and is important in breeding programs. The aim of this work therefore was to evaluate the similarity and genotype patterns which permit differentiation of the commercial radish cultivars IPR 116 and CATI AL-1000. In order to do this, isoenzyme electrophoretic patterns were analysed in dry seeds, soaked seeds, seedling leaves and young leaves; the isoenzymes used being: superoxide dismutase,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Raphanus sativus; ISSR; RAPD; Isoenzymes; Stage of development. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000400020 |
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| Mateus,Rogério Pincela; Cabral,Hamilton; Bonilla-Rodriguez,Gustavo Orlando; Ceron,Carlos Roberto. |
| A method that allows the measure of molecular weight of two well-known and closely related esterases from Drosophila mojavensis and its sibling species, D. arizonae, is here described, using native polyacrylamide gel electrophoresis at several concentrations, applying Fergunson´s principles. These enzymes, namely EST-4 and EST-5, presented molecular weight values between 81 and 91 kDa. In spite of their distinct expression pattern through the insect's life cycle, they showed properties of isoenzymes codified by distinct structural genes, supporting the hypothesis of a rather recent gene duplication event that generated both in D. mojavensis and D. arizonae, as well as in other species of repleta group. The method is simple and adequate to be applied to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Esterases; Isoenzymes; Molecular weight determination; Drosophila mojavensis; Drosophila arizonae; PAGE. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500004 |
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| Bonilla-Rivero,Ana; López-Rojas,Héctor. |
| We conducted a phylogeographic analysis of the genus Gephyrocharaxin Venezuela to evaluate geomorphologic evidence for the formation of the country's main watersheds and to establish a biogeographical hypothesis of possible diversification mechanisms of the Neotropical freshwater fish fauna. We assayed eight enzyme systems and general proteins to estimate genetic variability (H, P), intraspecific structuring in several Gephyrocharax valencia and G. venezuelae populations (FIS, FIT, and FST), and a phylogenetic approach for the three species of Gephyrocharax in Venezuela, using Corynopoma riisei as the external group. Fourteen presumptive lociindicate that populations of the three species of Gephyrocharaxanalyzed show a clear genetic inter-specific... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Isoenzymes; Speciation; Venezuela; Vicariance. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300487 |
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| Kopp,Rogério Luiz; Thomaz-Soccol,Vanete; Klisiowicz,Débora do Rocio; Membrive,Norberto; Barata,José Maria Soares; Jurberg,José; Steindel,Mário; Kopp,Denize Cristina Trevisan; Castro,Edilene Alcântara de; Luz,Ennio. |
| Panstrongylus megistus is an important Chagas Disease vector and is said to be one of the species that might replace Triatoma infestans as the main vector of that disease in Brazil. The different degrees of P. megistus domiciliation in Brazil and its epidemiological relevance draw forth the need for the development of genetic studies that make it possible to analyze and understand the interchange of individual and gene fluxes among different populations. Thus, the present work aimed at studying the genetic variability of P. megistus in the State of Paraná - south of Brazil- and at comparing it with populations of the same species from five other states in Brazil (SP, MG, SC, RS, SE). In order to attain the proposed objective, 25 populations were studied... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Panstrongylus megistus; Isoenzymes; Genetic variability; Triatomines. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200012 |
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| Baliza,Danielle Pereira; Caixeta,Franciele; Pinho,Édila Vilela de Resende Von; Cunha,Rodrigo Luz da; Martins,Denize Carvalho; Rosa,Sttela Dellyzete Veiga Franco da. |
| In Brazil, although the coffee plantations are predominantly grown under full sunlight, the use of agroforestry systems can lead to socioeconomic advantages, thus providing a favorable environment to the crop by promoting its sustainability as well as environmental preservation. However, there is a lack of information on physiological quality of the coffee seeds produced under different levels of solar radiation. Within this context, the objective of this study was to evaluate the influence of different levels of solar radiation and maturation stages on the physiological quality of coffee (Coffea arabica L.) seeds, cv. Acaiá Cerrado MG-1474. Three levels of solar radiation (plants grown under full sunlight; under plastic screens of 35% shading; and under... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea arabica; Germination; Vigor; Isoenzymes. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000300008 |
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| Ramírez-Avila,J.; Quevedo,B. E.; López,E.; Renjifo,J. M.. |
| Components with phospholipase A2 activity were isolated by gel filtration and cationic exchange chromatography from the venom of Bothrops asper snakes from Chiriguaná, Colombia (9°22´N; 73°37´W). Five fractions were obtained by the gel filtration, and PLA2 activity was found in fraction 3 (F3). In the cationic exchange chromatography, F3 showed eight components with PLA2 activity. Six of these components appeared as one band in polyacrylamide gel electrophoresis (SDS-PAGE). Fractions II and VII exhibited an optimal activity at pH 9 and 52ºC. The optimum calcium concentration for fraction II was 48 mM and for fraction VII, 384 mM. Both fractions showed thermal stability. Fraction II was stable at pH values between 2.5 and 9, and fraction VII, between 2.5... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phospholipase; Isoenzymes; Snake; Venom; Bothrops asper; Colombia. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300005 |
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| Galante,Rafaela S.; Taranto,Alex G.; Koblitz,Maria G.B.; Góes-Neto,Aristóteles; Pirovani,Carlos P.; Cascardo,Júlio C.M.; Cruz,Sandra H.; Pereira,Gonçalo A.G.; Assis,Sandra A. de. |
| The enzyme chitinase from Moniliophthora perniciosa the causative agent of the witches' broom disease in Theobroma cacao, was partially purified with ammonium sulfate and filtration by Sephacryl S-200 using sodium phosphate as an extraction buffer. Response surface methodology (RSM) was used to determine the optimum pH and temperature conditions. Four different isoenzymes were obtained: ChitMp I, ChitMp II, ChitMp III and ChitMp IV. ChitMp I had an optimum temperature at 44-73ºC and an optimum pH at 7.0-8.4. ChitMp II had an optimum temperature at 45-73ºC and an optimum pH at 7.0-8.4. ChitMp III had an optimum temperature at 54-67ºC and an optimum pH at 7.3-8.8. ChitMp IV had an optimum temperature at 60ºC and an optimum pH at 7.0. For the computational... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chitinase; Moniliophthora perniciosa; Kinetic characterization; Purification; Isoenzymes; Heat stability; 3D structure; Comparative modeling. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200016 |
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| Registros recuperados: 20 | |
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